Fig. 3: Layer 1, GM and WM astrocytes display unique molecular features.
From: Astrocyte heterogeneity reveals region-specific astrogenesis in the white matter
a, Heatmap showing the expression of 150 DEGs between layer 1, GM and WM astrocytes (Pval < 0.05, log2 fold change > 1). Two-sided Mann–Whitney U-test with Bonferroni post-hoc test. b, t-SNE visualization showing the expression levels (normalized) of two genes highly expressed in GM (Gria2 and Slc7a10, top) and in WM astrocytes (Vim and Lima1, bottom). c, Expression levels of the same genes shown in b on the spatial dataset showing their expression (Gria2 and Slc7a10 in the GM, and Vim and Lima1 in the WM). d, RNAscope in situ hybridization of Gria2, Slc7a10, Vim and Lima1. These results show enriched expression of Gria2 and Slc7a10 in GM astrocytes and enriched expression of Vim and Lima1 in WM astrocytes. n = 4 animals (for Vim staining in layer 1 n = 2). Scale bars, 400 μm(overview) and 10 μm (insets). e, GO term (biological process) analysis using genes enriched in WM and GM astrocytes. f, Radar plot comparing gene scores for six chosen functions across layer 1, GM and WM astrocytes. The genes used to calculate these scores were obtained from six corresponding GO terms. The graphs display the mean ± s.e.m.
