Fig. 4: A subset of WM astrocytes exhibit proliferative capacities.
From: Astrocyte heterogeneity reveals region-specific astrogenesis in the white matter
a, t-SNE visualization of the two WM clusters (clusters 4 and 5). b, DEGs between cluster 4 and 5. The red dots represent genes that are differentially expressed between the two clusters (Pval < 0.01, log2 fold change > 0.35), whereas genes expressed by both WM astrocyte subtypes are represented by black dots. Two-sided Mann–Whitney U-test with Bonferroni post-hoc test. c, The Vizgen MERSCOPE single-cell spatial dataset showing expression of Gfap and Ascl1 in astrocytes from three coronal sections (rostral, medial and caudal) in the WM. Right, Quantifications of the fraction of astrocytes positive for Gfap or Ascl1 in GM and WM. n = 3 sections (one rostral, one medial and one caudal). The graphs display the mean ± s.e.m. d, GO term (biological process) analysis using genes enriched in clusters 4 and 5. e, t-SNE visualization and violin plots of the proliferation score in both WM clusters. For the complete gene list used for the proliferation score, see Supplementary Table 1. f, t-SNE visualization of clusters 4 and 5 showing the expression levels (normalized) of some proliferation genes used for the proliferation score.
