Fig. 5: Profile of cerebellar WM astrocytes shows region-specific hallmarks.

a, t-SNE visualization of scRNA-seq data from the WM of the cerebellum visualized according to cell type. b, t-SNE visualization showing the astrocyte score and expression levels (normalized) of common markers for astrocytes. c, Cell type scores used to annotate the scRNA-seq dataset in a. For the complete gene list used for each cell type, see Supplementary Table 1. d, t-SNE visualization of the astrocytes identified and subset from a segregated in six different clusters. e–g, Annotation of the cerebellar layers on a sagittal brain section (e) and spatial mapping of the six cerebellar astrocyte clusters showing their predicted localization (f), which was used to annotate the six different clusters (g). h, t-SNE visualization of the integrated dataset of WM astrocytes from the WM/CC (Fig. 4a, clusters 4 and 5) and cerebellum (g), showing the region of origin (left) and the cluster analysis (right). i, Proportion of WM astrocytes in the clusters originating from the two regions (WM/CC versus cerebellum). j, t-SNE visualization of the integrated dataset showing the proliferation score.