Fig. 6: Human WM astrocytes share gene expression pattern with their mouse counterparts.
From: Astrocyte heterogeneity reveals region-specific astrogenesis in the white matter
a, t-SNE visualization of snRNA-seq data from the human WM visualized according to cell type. b, t-SNE visualization showing the cell type scores used to annotate the snRNA-seq dataset. For the complete gene list used for each cell type, see Supplementary Table 1. c, t-SNE visualization of astrocytes identified and subset from a segregated into two different clusters. d, Annotation of a human section showing the localization of the WM and the different GM cortical layers. e, Spatial mapping of the two human astrocytes clusters (0 and 1) showing their predicted localization. f, t-SNE visualization showing expression levels (normalized) of some genes highly expressed by WM astrocytes identified in the mouse dataset. g, Confocal images of human WM and GM double-stained for S100A6 and GFAP. Scale bars, 50 μm (overview) and 20 μm (insets). h, t-SNE visualization showing the proliferation score (left) and the prediction score with density distribution (right) for murine clusters 4 and 5 of WM astrocytes. The density distribution plot highlights areas of higher and lower concentration of cells displaying high and low levels of prediction scores in the dataset.
