Fig. 5: nAChR antagonism in DLS in vivo promotes DA release and conditioned place preference.
From: An axonal brake on striatal dopamine output by cholinergic interneurons
a, Schematic of configuration of FCV recording and stimulation electrodes in DLS. b, Color plots and representative line plots of oxidation current (from dashed yellow row at approximately +0.7 V) of voltammetric DA current versus time and a corresponding DA cyclic voltammogram (from time point of dotted black line) before and after mec (i.p.). c, DA signals (mean ± s.e.m.) evoked by one pulse or ten pulses (10p) at 100 Hz before (black) and after (red) mec (2 mg kg−1 i.p.). d, Mean peak DA currents (± s.e.m.). **P = 0.0340 for two-sided Šidák’s post-hoc test with multiple comparisons adjustment (N = 4 animals). e, The schematic of configuration of optic-fiber photometry recording in DLS and stimulation in SNc. f, Example traces of fiber photometry recording of GRABDA2m signal with local injection of DHβE (0.07 µg in 200 nl saline) or saline (200 nl) showing an elevation in baseline levels of non-evoked [DA]o after DHβE only. Intermittent electrical stimulation (nine pulses (9p) at 40 Hz elec, red lines) of SNc was applied at 0.05 Hz as a reference. g, The mean maximal change in baseline tonic DA (± s.e.m.) after local injection of DHβE or saline control. ***P = 0.0001, two-sided paired t-test (n = 5 from N = 3 animals). h, Schematic illustration of a bilateral cannula system for local infusion to dorsal striatum, and an example hemislice with DAPI staining. i, Conditioning paradigm. Mice were conditioned for four times over 2 days (20 min per session) after local infusion of mec (10 µg per side, right chamber, red) or saline (0.5 µl over 1 min, left chamber, black), whereas the control groups received saline for both chambers. j, Representative tracking traces from the postconditioning day. k, Preference for the right chamber after conditioning (± s.e.m.). *P = 0.0451, two-sided paired t-test, N = 5 animals in each test. l, Total travel distances and velocity of movements (± s.e.m.) in open-field test (N = 5 animals).
