Extended Data Fig. 1: Histology and immunostaining for GCaMP6f and GAD67.
From: Spatially clustered neurons in the bat midbrain encode vocalization categories
a, GFP immunostaining shows expression of GCaMP6f throughout the IC. The black box indicates the location of the site highlighted in b. b, Immunostaining for IC locus highlighted in a for cell nucleus (DAPI, top left), GCaMP6f (GFP, top right), inhibitory neurons (GAD67, bottom left), and merged image (bottom right). Merged image suggests minimal overlap between GCcAMP6f and GAD67 expression. Example neurons numbered on the merged image are displayed on the right: 1) GAD67+ and GcAMP6f+ (yellow), 2) GcAMP6f+ and GAD67- (green), 3) GcAMP6f- and GAD67+ (red). The staining procedure was repeated on the brains of two bats.
