Extended Data Fig. 2: Site depth estimation corrected by skull thickness.
From: Spatially clustered neurons in the bat midbrain encode vocalization categories
a, Mean images (150 frames) from 1X volumetric stack for example 2X imaging site (highlighted by the black to gray box, colors correspond to depth in b) every 10 µm until example site imaging depth (bottom image). b, Raw fluorescence distribution for images displayed in A. Note that shallower images possess a longer high fluorescence tail indicating a larger skull coverage. The criterion used for skull identification (95th percentile of the overall volumetric stack distribution) is displayed in the dashed line. c, Skull thickness and corresponding depth estimation for example volumetric stack in a and b.
