Extended Data Fig. 8: MERSCOPE gene panel analysis of FCDII postoperative brain tissues.
From: Single-cell genotyping and transcriptomic profiling of mosaic focal cortical dysplasia
a, Heatmaps showing normalized (SCTransform) gene expression levels of the 140-gene MERSCOPE panel, grouped by category. Abbreviations: DN, dysmorphic neurons; BC, balloon cells. b, Representative MERSCOPE images of top expressed genes (from Fig. 4h) in DN (pS6+/NEUN+) and BC (pS6+/NEUN-) from patient pt9 (n = 1 tissue section examined). Yellow puncta indicate individual transcript molecules. Scale bar = 50μm. c, Spatial detection of mitochondrial gene transcripts (yellow puncta) in DN from patients pt5 and pt9 (n = 1 tissue section examined for each patient). Scale bar = 50 μm.
