Fig. 6: MBNL and SRRM regulate the same miEs.
a, Age and sex distribution of ASD and CTRL prefrontal cortex samples in PsychENCODE RNA-seq data. ASD was confirmed by the Autism Diagnostic Interview-Revised (n = 8) or supported by records (n = 2). Box plot shows the lower, middle and upper quartiles. Whiskers show minimum and maximum. Two-tailed t-test; NS, P = 0.83. b, The number and percentage of AS event types significantly mis-spliced in ASD. c, ANK2 miE mis-splicing in ASD. Sashimi plots of ASD (n = 8) and CTRL (n = 8) RNA-seq. #FDR = 0.056. d, The number and percentage of AS event types significantly mis-spliced in Srrm DKD N2a cells (n = 2). e, Ank2 miE mis-splicing in Srrm DKD RNA-seq (n = 2). f, Overlapping mis-spliced miEs in Srrm DKD CAD and Mbnl DKD N2a cells. g, Selected miE splicing analysis in Mbnl DKD, Srrm DKD and Mbnl;Srrm QKD N2a cells (n = 4). h, Capillary electrophoresis analysis of Ank2 miE and A3SS. CI, combination index (g,h). One-way ANOVA followed by Tukey’s multiple comparisons test; NS, Padj > 0.05, *Padj < 0.05, **Padj < 0.01, ***Padj < 0.001, ****Padj < 0.0001. Data are presented as mean ± s.d (c,g–h).
