Fig. 8: Social behavior deficits in two DM1 mouse models.
a, Scheme of the three-chamber habituation and sociability test. b,e, Ratio of time the test mice spent in the chamber with a novel animal (stranger) and object during the sociability test. Time spent in the middle chamber is not included. b, WT mice (n = 11), Dmpk-(CTG)480/WT (n = 11), and Dmpk-(CTG)480/480 (n = 11). Paired two-tailed t-test; NS, P = 0.38 (t = 0.9134, d.f. 10), *P = 0.013 (t = 2.997, d.f. 10), **P = 0.0025 (t = 4.012, d.f. 10). c, Representative mouse movement during the habituation phase. d, Normalized mean values for 13 parameters measured in the automated open field test. WT mice (n = 11), Mbnl2 KO (n = 12). Two-tailed t-test; NS, P = 0.83 (t = 0.2131, d.f. 21). e, WT mice (n = 12), Mbnl2 KO (n = 12); two-tailed t-test; NS, P = 0.43 (t = 0.8193, d.f. 11), *P = 0.023 (t = 2.641, d.f. 11). f, Scheme of the free dyadic social interaction test. g, Representative mouse interactions. h, Mouse interactions analysis. WT (n = 15), Mbnl2 KO (n = 14). Interaction time and brief interaction; two-tailed t-test; ***P = 0.00098 (t = 3.696, d.f. 27), ****P < 0.0001 (t = 9.842, d.f. 27). Average length; two-tailed Mann–Whitney U-test; ****P < 0.0001. i, Scheme of the three-chamber social novelty test. Ratio of interactions with novel animal (stranger 2) and familiar animal (stranger 1) during the social novelty test. WT mice (n = 12), Mbnl2 KO (n = 12). Paired two-tailed t-test: NS, P = 0.078 (t = 1.941, d.f. 11), *P = 0.025 (t = 2.594, d.f. 11). j, Representative photos of nestlet shredding. WT (n = 17), Mbnl2 KO (n = 16); two-tailed Mann–Whitney U-test; ****P < 0.0001. Box plot shows the lower, middle and upper quartiles (b,d,e,h–j). Whiskers show minimum and maximum. k, Molecular mechanism of autism associated with myotonic dystrophy.
