Extended Data Fig. 3: Activity of VTA^DA neurons following METH infusion between dominants and subordinates.

a, Schematic of the virus strategy and a representative image showing GCaMP expression and optic fiber placement (top). Scale bar = 200 µm. Diagrams showing viral expression and optic fiber location in the VTA for the fiber photometry experiments (bottom). b–e, Activity of VTA^DA neurons following i.v. injection of saline (b), 0.1 mg kg⁻¹ (c) and 0.3 mg kg⁻¹ METH (d) and sucrose licking (e) in mice (saline and 0.1 METH: n = 5/group; 0.3 METH: sub, n = 4; dom, n = 3; sucrose: sub, n = 5; dom, n = 4). Mann–Whitney test. f, Schematic of the viral strategy to specifically record the NAc-projecting VTA neurons and a representative image (top). Scale bar = 200 µm. Diagrams showing viral expression and optic fiber location in the VTA for the fiber photometry experiments (bottom). g,h, Activity of NAc-projecting VTA^DA neurons following i.v. injection of 0.1 mg kg⁻¹ (g) and 0.3 (h) mg kg⁻¹ METH in mice (sub, n = 5; dom, n = 6). Two-sided unpaired t test. Each row in the heatmap represents averaged DA dynamics over trials from individual mouse (ranked by AUC). Data are represented as mean ± s.e.m. Detailed statistical information is available in Supplementary Table 1.

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