Extended Data Fig. 9: Both female groups exhibited high METH-seeking behavior.
a, Timeline and schematics showing the tube test and METH self-administration in female rats. b, Winning probability across trials and total wins in female rats (n = 8 pairs). Two-sided paired t test, ****P < 0.0001. c,d, Behavioral outcome in the warm spot test (c) and agonistic behavior (d) for females (n = 12 mice per group). Two-sided paired t test, *P < 0.05. e–g, Nose pokes (e) and infusions (f) across sessions and average number (g) during the last three days in female (n = 5 per group) and male rats (n = 10 per group). e, Two-way ANOVA, *P < 0.05, followed by Tukey’s test; f, **P < 0.01, followed by Tukey’s test, ****P < 0.0001. g, One-way ANOVA, **P < 0.01, followed by Sidak’s test, ***P < 0.001. h, Timeline of the fiber photometry recordings in females. i,j, Schematic (i) and verification (j) of viral injections and optic fiber placement in the mPFC. Scale bar = 0.5 mm. k,l, DA release in the mPFC following 0.1 mg kg−1 (k) and 0.3 mg kg−1 (l) METH infusion in female (0.1 METH: sub, n = 9; dom, n = 8; 0.3 METH: n = 8/group) and male (0.1 METH: sub, n = 9; dom, n = 8; 0.3 METH: sub, n = 9; dom, n = 10) mice. Two-sided unpaired t test, *P < 0.05, **P < 0.01. m, Schematic (m) and verification (n) of viral injections and optic fiber placement in the NAc. Scale bar = 0.5 mm. o,p, DA release in the NAc following 0.1 mg kg−1 (o) and 0.3 mg kg−1 (p) METH infusion in female (0.1 METH: sub, n = 9; dom, n = 8; 0.3 METH: n = 8 per group) and male (0.1 METH: sub, n = 13; dom, n = 14; 0.3 METH: sub, n = 9; dom, n = 8) mice. Two-sided unpaired t test, *P < 0.05, **P < 0.01. Each row in the heatmap represents averaged DA dynamics over trials from individual mouse (ranked by AUC). Data are represented as mean ± s.e.m. Detailed statistical information is available in Supplementary Table 1.
