Extended Data Fig. 10: The aging human brain has cytoplasmic TDP-43 foci.
From: Neuronal aging causes mislocalization of splicing proteins and unchecked cellular stress
a, Confocal immunofluorescence images of frontal cortex tissue from aged cohorts of human brains. Green is Map2, magenta is TDP-43 and blue is DAPI. White scale bar = 10 μm. The TDP-43 images are reproduced from Fig. 6d. b, Western blot of eIF2α and phospho-eIF2α in aged human brain samples (N = 3 samples per cohort). Quantification is provided in Fig. 6f. c, Upset plot of shared 3′ UTR binding sites (enrichment score >3) among the indicated G3BP1 eCLIP samples. UTRs were grouped by genes. The orange bar indicates the 3′ UTRs that were detected in all stressed samples, gray bars are other combinations of 3′UTRs detected in multiple genes and white bars denote 3′UTRs that were detected in only one sample. d, Representative genomic tracks of G3BP1 binding to the MAPT 3′ UTR in cultured neurons (top three tracks, original data in Fig. 3) and brain tissue (bottom two tracks, original data in Fig. 6). The exons and 3′UTR are indicated on the gene.
