Extended Data Fig. 10: Characterization of motor responses to long-term (16 h) opto-stimulation.
From: Activity-driven myelin sheath growth is mediated by mGluR5
A: Long-term opto-stimulation of the chx10 pre-motor interneuron circuitry followed by assessment of motor response. The opto-stimulation was carried out identically to the experiments in Fig. 6. Control siblings were kept in a 96-well plate in the dark. After long-term opto-stimulation of chx10:ChRimsonR, the effects of acute activation were tested by assessing the latency between the onset of fiber illumination and initiation of the swimming movement. B: Optical stimulation of a control animal, showing induction of the swimming movement (asterisk). C: Optical stimulation of an animal following 16 h of long-term opto-stimulation, showing induction of swimming movement (asterisk). D: Mean latency between opto-stimulation and motor response in controls and animals that had undergone 16 h of long-term opto-stimulation. E: Latency from the first opto-stimulation to motor response per animal (Control N = 20, mean: 127.5 SD: ±54.86; Opto-stimulated N = 33, mean: 77.27, SD: ±29.71) (Mann–Whitney test; p-value = 0.0003).
