Extended Data Fig. 2: mGluR5 loss of function oligodendrocytes have shorter myelin sheaths and are growing between 3 and 4 dpf.

A: 3 and 4 dpf wild-type oligodendrocytes labeled with mpb:eGFP-CAAX (N = 36). B: 3 and 4 dpf grm5a^-/-grm5b^-/- oligodendrocytes at 3 dpf (N = 22). C: 3 and 4 dpf grm5a^-/- oligodendrocytes (N = 14). D: 3 and 4 dpf grm5b^-/- oligodendrocytes (N = 17). E: Relative frequency distribution of individual measured myelin sheath lengths in 3 dpf control and loss of function mutants (WT n = 583, mean: 20.03 µm) (grm5a^-/- n = 259, mean: 14.08 µm) (grm5b^-/- n = 279 mean: 13.48 µm) (grm5a^-/-grm5b^-/- n = 338, mean: 15.89 µm). F: Relative frequency distribution of individual measured myelin sheath lengths in 4 dpf control and loss of function mutants (WT n = 288, mean: 27.52 µm; grm5a^-/- n = 164, mean: 15.9 µm; grm5b^-/- n = 209, mean: 19.72 µm; grm5a^-/-grm5b^-/- n = 191, mean: 18.88 µm). G Relative frequency distribution of individual measured myelin sheath length in 7 dpf WT (n:212 mean: 31.34 µm) and grm5a^-/-grm5b^-/- (n = 259, mean: 21.87) animals. H: Comparison between sheath lengths per cell in 3 and 4 dpf fish (One-Way ANOVA p-value > 0.0001; Tukey’s multiple comparisons test: 3 dpf WT vs. 3 dpf grm5a^-/- p-value = 0.0038; 3 dpf WT vs. 3 dpf grm5b^-/- p-value = 0.0064; WT 3 dpf vs. 3 dpf grm5a^-/- grm5b^-/-p-value = 0.0293; 3 dpf WT vs. 4 dpf WT: p-value = <0.0001; WT 4 dpf vs. 4 dpf grm5a^-/- p-value = <0.0001; WT 4 dpf vs. 4 dpf grm5b^-/- p-value = <0.0028; WT 4 dpf vs. 4 dpf grm5a^-/-grm5b^-/- p-value = 0.0002; 3 dpf WT vs 4 dpf grm5a^-/-grm5b^-/- p-value = 0.9842; 3 dpf grm5a^-/- vs. 4 dpf WT p-value = <0.0001; 3 dpf grm5b^-/- vs. 4 dps WT p-value = <0.0001, 3 dpf grm5a^-/-grm5b^-/- vs. 4 dpf WT p-value = <0.0001). WT 3 dpf mean: 21.11, SD: ±5.15; 3 dpf grm5a^-/- mean= 14.5, SD: ±4.77; 3 dpf grm5b^-/- mean= 15,17 SD: ±5.38; 3 dpf grm5a^-/- grm5b^-/- mean= 16.34 SD: ±5.376; 4 dpf WT mean: 28.8, SD: ±6.519; 4 dpf grm5a^-/- mean: 15.99 SD: ±3.94, 4 dpf grm5b^-/- mean: 20.83 SD: ±6.716; 4 dpf grm5a^-/-grm5b^-/- mean: 19.47, SD: ±3.67). I: Myelin sheath numbers of 3 and 4 dpf WT and mGluR5 mutants (One-way ANOVA p-value = 0.2457 Tukey’s multiple comparisons test: 3 dpf WT vs. 3 dpf grm5a^-/- p-value = 0.8436; 3 dpf WT vs. 3 dpf grm5b^-/- p-value = 0.9995; WT 3 dpf vs. 3 dpf grm5a^-/- grm5b^-/-p-value = 0.9988; 3 dpf WT vs. 4 dpf WT: p-value = >0.9999; WT 4 dpf vs. 4 dpf grm5a^-/- p-value = 0.4523; WT 4 dpf vs. 4 dpf grm5b^-/- p-value = 0.982; WT 4 dpf vs. 4 dpf grm5a^-/-grm5b^-/-p-value = >0.9999; 3 dpf WT vs. 4 dpf grm5a^-/-grm5b^-/- p-value = 0.9992, 3 dpf grm5a^-/- vs. 4 dps WT p-value = 0.8797; 3 dpf grm5b^-/- vs. 4 dps WT p-value = 0.9994, 3 dpf grm5a^-/-grm5b^-/- vs. 4 dpf WT p-value = 0.9999). WT 3 dpf mean: 16.2, SD: ±4.80; 3 dpf grm5a^-/- mean: 18.5, SD: ±5.00 3 dpf grm5b^-/- mean: 17, SD: ±4.74; 3 dpf grm5a^-/- grm5b^-/- mean: 15.36 SD: ±3.94; 4 dpf WT mean: 16.06 SD: ±4.04; 4 dpf grm5a^-/- mean: 20.5 SD: ±8.23, 4 dpf grm5b^-/- mean: 17.83 SD: ±7.49; 4 dpf grm5a^-/-grm5b^-/- mean: 15.92, SD: ±4.46).

Source data