Extended Data Fig. 3: mGluR5 loss of function mutation does not alter myelin thickness, or nodal gap width.
From: Activity-driven myelin sheath growth is mediated by mGluR5
A: Confocal images of mbp:memGCaMP7s used to assess myelin thickness around the Mauthner axon in wild-type and grm5a-/- grm5b-/- at 3 and 7 dpf. B: Confocal images of mbp:memGCaMP7s indicating myelin sheaths and nodal gaps in wild-type and grm5a-/- grm5b-/- animals at 3 and 7 dpf. C: Myelin thickness in wild-type (N = 13, mean: 0.40 µm, SD: ±0.09) and grm5a-/- grm5b-/- (N = 10, mean: 0.41 µm, SD: ±0.11) animals at 3 dpf (Unpaired t-test: p-value = 0.8754). D: Myelin thickness in wild-type (N = 11, mean: 0.64 µm, SD: ±0.11) and grm5a-/- grm5b-/- (N = 9, mean: 0.71 µm, SD: ±0.07) animals at 7 dpf (Unpaired t-test: p-value = 0.1385). E: Avg. nodal gap per animal in wild-type (N = 13, mean: 1.49 µm, SD: ±0.40) and grm5a-/- grm5b-/- animals (N = 9, mean: 1.80 µm, SD: ±0.41) at 3 dpf (Unpaired t-test: p-value = 0.091). F: Avg. nodal gap per animal in wild-type (N = 6, mean: 1.09 µm, SD: ±0.26) and grm5a-/- grm5b-/- (N = 7, mean: 1.08 µm, SD: ±0.22) animals at 7 dpf (Unpaired t-test: p-value = 0.9618). G: Avg. Myelin sheath length per animal in wild-type (N = 13, mean: 21.71 µm SD: ±4.92) and grm5a-/- grm5b-/- (N = 9, mean: 16.11 µm SD: ±4.40) animals at 3 dpf (Unpaired t-test: p-value = 0.0128). H: Avg. Myelin sheath length per animal in wild-type (N = 13, mean: 24.57 µm, SD: ±3.08) and grm5a-/- grm5b-/- (N = 8 mean:15.81 µm, SD: ±1.64) animals at 7 dpf (Mann–Whitney test: p-value = 0.0002).
