Extended Data Fig. 9: Psilocybin bidirectionally modulates neural ensembles driving RSC dynamics during TFC in responders.

a, Overlaps of ensembles within individual animals comprising the mean values in Fig. 4b for the Acq- (top), Ext1- (middle) and Ext3-dominant (bottom) ensembles. b, Fisher decoder performance on Acquisition activity in functionally defined ensembles of cells to distinguish psilocybin groups (black), low-freezing psilocybin vs. saline (light purple) and high-freezing psilocybin vs. low-freezing saline mice (dark purple). Hundred iterations for each comparison. Shuffled values are behind real values. c,d, Three-way Fisher decoder performance low-freezing psilocybin vs. high-freezing psilocybin vs. low-freezing saline mice trained on activity during Extinction 1 (c) and Extinction 3 (d). e–g, Fisher decoder performance classifying psilocybin low- vs. saline low-freezing mice (e), psilocybin high- vs. saline low-freezing mice (f) and psilocybin groups (g) based on ensemble activity during Extinction 1 (top) and 3 (bottom) activity during freezing (salmon), motion (turquoise) and both (black). h–n, Average z-score activity in ensemble neurons in the Acq-only (h), Ext1-only (i), Ext3-only (j), Acq/Ext1 (k), Acq/Ext3 (l), Ext1/Ext3 (m), and Acq/Ext1/Ext3 (n) ensembles from Acquisition during freezing and motion in Extinction 1 (top) and Extinction 3 (bottom; multiple paired t tests for each ensemble within session, FDR = 0.01; Supplementary Table 9 (rows 18–31)). Stars indicate discoveries. Data displayed as mean ± s.e.m.

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