Extended Data Fig. 8: Exercise remodels AD-dysregulated pathways in mGCs.
a, Average size of amyloid-beta plaques (3D6 staining) in ventral DG sections (APP/PS1-Sed n = 5, APP/PS1-Run n = 6, three section per animal; Two-tailed unpaired t-test P = 0.6679). Data is represented by the median (middle bold line) and upper and lower quartiles (lighter dotted lines). b, Schematic representation of amyloid precursor protein (APP) processing and amyloid beta degradation pathways. Adapted from the KEGG pathway database. c, Expression level of chimeric mouse/human APPswe and the human PS1-dE9 transgene in the APP/PS1 mice, and the fraction of cells expressing the gene. d, Expression levels of alpha-secretase (Adam10), beta-secretase (Bace1), and gamma-secretase (Psenen, Ncstn, Aph1a) coding- genes in all neuronal clusters and the fraction of cells expressing the gene. e, Expression levels of the Aβ-degrading enzyme coding-gene Ide and Mme in all cell clusters and the fraction of cells expressing the gene. f, Immediate early gene expression in the different neuronal cell types by group. In each dotplot, the hue and size of the dot represent the mean expression and fraction of non-zero count nuclei, respectively. Data represented by biologically independent samples. Panel b created using BioRender.com.
