Fig. 2: Septal astrocytes display distinct lineage-specific molecular signatures.
From: Astrocyte specification in the mouse septum is shaped by both developmental origin and local signals
a, Experimental design for snRNA-seq on sorted cells from Nkx2.1Cre;Sun1GFP septum at different developmental stages. FANS, fluorescence-activated nuclei sorting. b, Uniform Manifold Projection and Approximation (UMAP) plot of cell types from all samples (n = 166,418 cells). c, UMAP plot of all septal astrocyte clusters. d, UMAP plot of septal astrocytes annotated by age. e, Proportion of cells at each age across clusters. f, UMAP plot of all GFP+ and GFP− astrocytes. g, Proportion of GFP+ and GFP− astrocytes in each cluster. h, UMAP plot of P21 septal astrocytes at a 0.3 resolution level. i, UMAP plot of P21 GFP+ and GFP− astrocytes. j, Heatmap showing the top 20 DEGs in LSAs and MSAs. k, MERSCOPE analysis of P35 septal astrocyte clusters along the rostrocaudal axis. l,m, MERSCOPE analysis showing gene enrichment in LSAs (l) and MSAs (m). n,o, MERSCOPE analysis showing representative genes enriched in LSAs (n) and MSAs (o) along the rostrocaudal axis. Icons in a created with BioRender.com. LSP1, lateral septal astrocyte progenitor cluster 1; LSP2, lateral septal astrocyte progenitor cluster 2; neg, negative; pos, positive; t-SNE, t-distributed stochastic neighbor embedding.
