Fig. 3: MSAs and LSAs display unique age-dependent molecular profiles.
From: Astrocyte specification in the mouse septum is shaped by both developmental origin and local signals
a,b, UMAP plot showing age-dependent MSA (a) and LSA (b) clusters. c,d, Heatmap showing the top-ten DEGs in GFP+ (c) and GFP− (d) astrocytes across ages. e–h, Heatmaps showing the top-ten DEGs enriched in GFP+ versus GFP− at P0 (e), P3 (f), P7 (g) and P14 (h). i, Representative genes enriched in GFP+ astrocytes during development. j, Representative genes enriched in GFP− astrocytes during development. k, Slc6a11 messenger RNA (mRNA) in P0–P14 septum of WT (CD1) mice detected using in situ hybridization. Subdivisions of the septum are indicated with white lines. Scale bar, 100 µm. l, Heatmap showing the relative density of puncta of Slc6a11 mRNA in each corresponding LS subnucleus, normalized by the density of puncta in the MS, at different ages (n = 3–5 mice for each age). m, Violin plots showing Slc6a11 expression (snRNA-seq) in both MSAs and LSAs at P0 and P14. The average expression levels are indicated. n, Slc1a2 mRNA evaluated in P0–P14 septum of WT (CD1) mice using in situ hybridization. Scale bar, 100 µm. o, Heatmap showing the relative density of puncta of Slc1a2 mRNA in each corresponding LS subnucleus, normalized by the density of puncta in the MS, at different ages (n = 3–5 mice for each age). p, Violin plots showing Slc1a2 expression (snRNA-seq) in both MSAs and LSAs at P0 and P14. The average expression levels are indicated.
