Fig. 3: Modulation of ILC subsets as a function of virtual or real stimulation.
From: Neural anticipation of virtual infection triggers an immune response
a, ILC subset distribution. Left, representative dot plots of ILC1s identified as CRTH2−cKit−, ILC2s as CRTH2+cKit+/− and ILCPs as CRTH2−cKit+ among total ILCs (that is, living lineage−CD127+ lymphocytes; see Fig. 2b) at baseline and after the second VR session. Right, changes in ILC1, ILC2 and ILCP frequencies among the different cohorts (ANOVA ILC1s: F3,56 = 4.69, P = 0.0054; ILC2s: F3,56 = 4.81, P = 0.0048; ILCPs: F3,56 = 3.65, P = 0.018). b, ILC activation by flow cytometry. Left, representative histograms of the percentage of CD25+ ILCs at baseline and after the second VR session as an illustrative example of ILC activation. Right, synthetic index of ILC1, ILC2 and ILCP activation changes (first PCA component) in the different cohorts (ANOVA ILC1s: F3,56 = 3.68, P = 0.017; ILC2s: F3,56 = 2.49, P = 0.07; ILCPs: F3,56 = 2.67, P = 0.056). In a and b, N = 15 distinct participants per cohort. Mean (dot) ± s.e.m. (line) is shown in black. Data are presented as the difference between the values after the second VR exposure and values at baseline.
