Extended Data Fig. 5: AAV-driven ISL1 and LHX3 expression are observed in gamma motor neurons. | Nature Neuroscience

Extended Data Fig. 5: AAV-driven ISL1 and LHX3 expression are observed in gamma motor neurons.

From: Embryonic motor neuron programming factors reactivate immature gene expression and suppress ALS pathologies in postnatal motor neurons

Extended Data Fig. 5

Representative immunostaining in the L4-L5 region of the ventral horn from animals injected with AAV-Isl1+AAV-Lhx3 (6.38E+10 total vg/animal) at P1 and analyzed at P14 (a) or P21 (b). Images are maximum intensity projections of 8 z sections taken 3 µm apart. Filled arrowheads indicate ectopic ISL1 and/or LHX3-expressing gamma motor neurons, identified as small-diameter CHAT+ cells that lack NEUN expression. Outlined arrowheads indicate gamma motor neurons that do not currently express ISL1 or LHX3. Scale bars represent 20 µM. Similar immunostainings were performed in n = 4 mice at P14 and n = 2 mice at P21.

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