Extended Data Fig. 2: Expression of lentivirus and AAV in the mouse brain. | Nature Neuroscience

Extended Data Fig. 2: Expression of lentivirus and AAV in the mouse brain.

From: CRISPR screening by AAV episome-sequencing (CrAAVe-seq): a scalable cell-type-specific in vivo platform uncovers neuronal essential genes

Extended Data Fig. 2

a, Sagittal section of mouse brain injected with sgRNA construct packaged in VSV-G-pseudotyped lentivirus (EF1a-NLS-mTagBFP2; pMK1334), stained with antibody recognizing mTagBFP2 at 3 weeks after ICV injection. Scale bar is 1 mm for low-magnification and 100 µm for subpanels (a1) and (a2). b, Sagittal section of mouse brain injected with sgRNA construct packaged in AAV PHP.eB capsid (EF1a-NLS-mTagBFP2; pAP215), stained with antibody recognizing mTagBFP2 at 3 weeks after ICV injection. This condition is sgCreb1 + hSyn1-Cre (mouse 2), used in Fig. 2 and in Extended Data Fig. 4. Scale bar is 1 mm for low magnification and 100 µm for subpanels (b1), (b2), (b3), and (b4).

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