Extended Data Fig. 3: Cre-dependent CRISPRi knockdown of CREB1 in different brain regions of a mouse. | Nature Neuroscience

Extended Data Fig. 3: Cre-dependent CRISPRi knockdown of CREB1 in different brain regions of a mouse.

From: CRISPR screening by AAV episome-sequencing (CrAAVe-seq): a scalable cell-type-specific in vivo platform uncovers neuronal essential genes

Extended Data Fig. 3

Immunofluorescent staining for CREB1 (red) in an LSL-CRISPRi mouse 3 weeks after injection with PHP.eb::pAP215-sgNTC or -sgCreb1 (blue) along with PHP.eB::hSyn1-Cre (green), for different brain regions (a, striatum; b, hippocampus; c, deep cerebellar nuclei; d, cerebellum Purkinje layer). Representative nuclei containing both BFP and GFP are outlined with a dotted circle, with the same nuclei shown in the top and bottom panels of each brain region, highlighting markedly reduced CREB1 in double-positive nuclei with sgCreb1 as compared to sgNTC. DG: dentate gyrus; ML: molecular layer, Pkj: Purkinje cell layer (Pkj); IGL: internal granule layer. Data is quantified in Extended Data Fig. 4b. Scale bars are 20 µm in a–d.

Back to article page