Extended Data Fig. 1: CrAAVe-seq enables simultaneously highly scalable and cell-type-specific in vivo screens. | Nature Neuroscience

Extended Data Fig. 1: CrAAVe-seq enables simultaneously highly scalable and cell-type-specific in vivo screens.

From: CRISPR screening by AAV episome-sequencing (CrAAVe-seq): a scalable cell-type-specific in vivo platform uncovers neuronal essential genes

Extended Data Fig. 1

a, Table summarizing published in vivo pooled screens of endogenous cells in the brain and AAV-based screen outside of the brain5,6,7,8,9,10,39. *Did not capture sgRNAs, instead performed mutagenesis screen; **murine genome-wide Asiago library; ***a specific brain region containing migrated neural stem cell (NSCs) provided selectivity. CRISPRko: CRISPR knockout. Highlighted cells are large-scale sgRNA libraries (green), cell-type selectivity by Cre (blue), and single-cell RNA-sequencing (scRNA-seq, red). b, Table comparing different aspects of AAV and lentivirus. Virus diagrams in panel b are created with BioRender.com. c, Simplified diagram of the hSyn1-Cre plasmid construct (Addgene, 105540). Panel c is created with BioRender.com.

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