Extended Data Fig. 8: miR-126-5p enhances neuronal growth of SOD1G93A primary motor neurons.
From: Muscle-derived miR-126 regulates TDP-43 axonal local synthesis and NMJ integrity in ALS models
a–c) Representative phase images with axonal marking (a) and quantification of neurite length (b) and cell-body cluster area (c) in WT, SOD1G93A or miR-126-GFP infected-SOD1G93A primary motor neurons at 3DIV. Blue, red and green segmented lines indicate the neurite tracing. Scale bar: 100 µm. Data are shown as the mean neurite length/cell body cluster area (mm/mm2) ± SEM (c), or cell body cluster area (area/mm2) ± SEM (d). n = 10; 15; 15 wells (over 6,000 neurons analyzed in every condition). One-way ANOVA with Holm-Sidak correction, **p = 0.00942(b), ****p = 0.00002(b), p = 0.1065(b), ****p = 5.41×10−8(c), *p = 0.0467. d) Representative image of pLV-miR-126 GFP infected primary motor neurons cultured in the proximal compartment of microfluidic chambers. Green indicates GFP. Scale bar: 100 µm.
