Extended Data Fig. 7: L2/3 neuronal activity changes induced by local serotonergic receptor modulation in ACC.
a, Representative GCaMP6f traces of individual neurons under wakefulness and following local (L-) psilocin (green-shaded area) at different doses (0 µM, 1 µM, 100 µM, 500 µM). Summary shown in Fig. 4h. b, L2/3 peak calcium responses in sham and SNI mice following saline and L-psilocin (200 µM; sham saline, n = 143 from 3 mice; sham L-psilocin, n = 144 from 3 mice; SNI sham, n = 194 from 3 mice; SNI L-psilocin, n = 276 from 4 mice). SNI condition increased the L2/3 peak responses compared to sham, which was reversed by L-psilocin (one-sided Kruskal–Wallis (98), P = 3.4 ×10−21 followed by Dunn’s multiple comparison: sham versus SNI, P = 7.6 × 10−11; sham saline versus sham L-psilocin, P = 5.2 ×10−4, SNI saline versus SNI L-psilocin, P = 1.3 × 10−19). c, L2/3 peak calcium responses in sham and SNI mice following vehicle control (saline) and local application of DOI (sham n = 189 from 4 mice, SNI n = 222 from 4 mice), 8-OH-DPAT (sham n = 100 from 3 mice, SNI n = 126 from 3 mice), or DOI + 8-OH-DPAT (sham n = 224 from 5 mice, SNI n = 171 from 5 mice). Drug concentration was 200 µM for each condition; one-sided Kruskal–Wallis (430), P = 9.3 × 10−89 followed by Dunn’s multiple comparisons shown in c. Data are represented as mean ± s.e.m in b,c.
