Extended Data Fig. 4: Dopamine signaling to the CDNs through the D2R supports mating persistence.
From: Behavioral devaluation by local resistance to dopamine
(A) Of the four dopamine receptors in Drosophila, whole-animal deletion of the D2 receptor most strongly increases termination in response to heat threats (n = 27-33). Note: we observed a courtship deficit and low mating rate in D2R mutant flies when conducting these experiments. We had not previously detected a role for this gene in courtship using a p-element insertion5,17. (B) Mutation of any of the four known dopamine receptors in Drosophila males has little effect on copulation duration (n = 10-15). (C) Electrical silencing of the dopamine neurons increases termination in response to a heat threat, an effect that is blocked by CDN silencing (n = 26-33). (D) Deletion and rescue of D2R expression in the CDNs does not alter copulation duration (n = 19-36). (E) Expression of UAS-D2R in the CDNs does not decrease the fraction of mating pairs terminating in response to a heat threat delivered at 10 minutes (n = 27-32). (F) RNAi-mediated knockdown of D2R in the CDNs does not alter copulation duration (n = 12-17). (G) Knockdown of the Dop1R1, which is expressed in some but not all CDNs, also increased termination in response to a heat threat at 8 mins into mating (n = 24-30).
