Extended Data Fig. 7: Assessment of dopaminergic signaling in satiated animals.
From: Behavioral devaluation by local resistance to dopamine
(A) Dopaminergic stimulation becomes less effective at extending matings after males spend ~2.5 hours mating with females in the satiety assay (n = 18-21). Note: the 2.5 hour time point is reproduced from Fig. 3a. (B) Basal activity of dopamine neurons in the abdominal ganglion does not change with satiety state, as reported by normalizing GCaMP6s to tdTomato fluorescence (n = 6-9). (C) Optogenetically evoked calcium activity in abdominal ganglion dopamine neurons does not change with satiety state (n = 4-6). (D) Recent matings do not change stimulation-induced dopamine received by the CDNs, as reported by GRAB-DA3m (n = 7-8). (E) Full trace of Fig. 3b, left panel: CDN calcium signal does not differ between not satiated and satiated animals (n = 19-20). (F) CDN calcium signal is not significantly depressed by 10 µM DA (n = 10-11). (G) Full trace of Fig. 3b, center left panel: CDN calcium signal is less depressed by bath application of 25 µM dopamine in satiated animals (n = 17-20). (H) Full trace of Fig. 3b, center right panel: CDN calcium signal is less depressed by bath application of 50 µM dopamine in satiated animals (n = 20 each). (I) CDN calcium signal is depressed upon bath application of 100 µM dopamine in not satiated and satiated animals (n = 10 each). (J) Left: schematic of the CaMKII sensor green-Camuiα. Right: Individual traces corresponding to Fig. 3c, left panel: saline application does not increase CaMKII activity in not satiated or satiated flies (n = 10 each). (K) Individual traces corresponding to Fig. 3c, middle panel: bath application of 10 µM dopamine increases CaMKII activity more strongly in not satiated than satiated flies (n = 19-20).
