Extended Data Fig. 8: Activation of Gαo signaling in the CDNs protects matings in real-time.
From: Behavioral devaluation by local resistance to dopamine
(A) Knockdown of Gαo in the CDNs does not change copulation duration (n = 14-18). (B) Full trace of Fig. 4c, left panel, CDN calcium signal is depressed upon bath application of 100 µM dopamine in control animals (n = 10 each). (C) Full trace of the data shown in Fig. 4c, middle panel, CDN calcium signal is only slightly depressed upon bath application of 100 µM dopamine in animals with Gαo knocked down in the CDNs (n = 10 each). (D) Similar to controls, males experiencing optogenetic activation of eOPN3 in the CDNs avoid temperatures above 28°C when not mating (n = ~10/group, 3-6 groups per test temperature).
