Extended Data Fig. 3: Simultaneous paired fiber photometry recordings of intracellular Ca2+ from the somata of STN neurons and their axonal terminals in the SNr of normal mice during high-frequency STN DBS.
From: Differential synaptic depression mediates the therapeutic effect of deep brain stimulation
a, Schematic illustration showing bilateral microinjections of AAVs to express GCaMP6f and tdTomato in STN neurons. b, Schematic illustration of simultaneous paired fiber photometry recordings in the STN and in the SNr during STN DBS. c, e, Time-lapsed normalized ratio of GCaMP6f/tdTomato fluorescence (plotted as mean ± SEM) to show the STN DBS-induced intracellular Ca2+ in the somata of STN neurons (c) and their axon terminals in the SNr (e). d, f, Summary of DBS-induced changes in the fluorescence ratio of GCaMP6f/tdTomato (normalized to the baseline before the stimulation) in the somata of STN neurons (d) and their axon terminals in the SNr (f). *** p < 0.001 (For d, p = 0.0006; for f, p = 0.0008), paired two tailed t test, n = 6 from 5 mice (one mouse received bilateral implantation with one pair of probes in each hemisphere), including both males and females.
