Extended Data Fig. 6: Effects of high-frequency STN DBS on negative controls of glutamate and GABA sensors in normal mice.
From: Differential synaptic depression mediates the therapeutic effect of deep brain stimulation
a, g, Schematic illustrations showing the microinjection of AAVs to express cpSFVenus (negative control for SF-Venus-iGluSnFR) with tdTomato (a) and cpSFGFP (negative control for iGABASnFR) with tdTomato (g) in STN neurons. b, h, Representative images to show the expression of cpSFVenus and tdTomato (b) and cpSFGFP and tdTomato (h) in STN neurons. Scale bars: 200 μm. c, i, Illustrations of fiber photometry recordings of local cpSFVenus (c) and cpSFGFP (i) co-expressed with control fluorescent protein tdTomato. d, j, Representative emission spectra of cpSFVenus and tdTomato (d) and cpSFGFP and tdTomato (j) expressed by STN neurons before and during DBS stimulation. e, k, Time-lapsed fluorescence ratio of cpSFVenus /tdTomato (e) and cpSFGFP/tdTomato (k), normalized to the baseline before the first DBS stimulation, to show the DBS-induced changes at different stimulation intensities. f, l, Summary of DBS-induced changes in fluorescence ratio of cpSFVenus /tdTomato (f) and cpSFGFP/tdTomato (l) in the STN at different stimulation intensities. ** p < 0.01 (For f: 100 µA vs. 150 µA, p = 0.0019), Repeated one-way ANOVA followed by Dunnett’s multiple comparisons test. All data are plotted as mean ± SEM. n = 9 from 7 mice (two mice had bilateral implants), including both males and females, for cpSFVenus group; n = 13 from 7 mice (6 mice had bilateral implants), including both males and females, for cpSFGFP group.
