Extended Data Fig. 8: Lack of effect of low-frequency (20 Hz) unilateral STN DBS on behavior, intracellular Ca2+ levels in STN neurons and afferent presynaptic terminals in the STN of ipsilaterally 6-OHDA-lesioned mice.
From: Differential synaptic depression mediates the therapeutic effect of deep brain stimulation
a, b, Rotation tests to compare the spontaneous rotations before and during low-frequency (20 Hz) STN DBS in sham-lesioned (a) and 6-OHDA-lesioned (b) Vglut2-cre mice. **** p < 0.0001 (For b: Baseline-Ipsilateral vs. Baseline-Contralateral, p < 0.0001; DBS-Ipsilateral vs. DBS-Contralateral, p < 0.0001), Two-way ANOVA followed by Tukey’s multiple comparisons test. All data are plotted as mean ± SEM. n = 10 mice for sham-lesioned group, n = 12 mice for 6-OHDA-lesioned group, including both males and females. c, f, Schematic illustrations showing the microinjection of AAVs to express GCaMP6f and tdTomato in STN neurons (c) and GCaMP7f in the afferent terminals in the STN and tdTomato in STN neurons (f). d, g, Time-lapsed fluorescence ratio (normalized to the baseline before the first DBS stimulation) of GCaMP6f/tdTomato in STN neurons (d) and presynaptic GCaMP7f/postsynaptic tdTomato in the STN (g) stimulated at different intensities with 20-Hz DBS. e, h, Summary of DBS-induced changes of the cytosolic Ca2+ in STN neurons (e) and afferent terminals in the STN (h) at different stimulation intensities with 20-Hz DBS. n = 5 mice for postsynaptic group (e) and n = 6 mice for presynaptic group (h), including both males and females, Repeated one-way ANOVA followed by Dunnett’s multiple comparisons test.
