Extended Data Fig. 9: Effect of unilateral 6-OHDA lesion on intracellular Ca2+ levels in STN neurons.
From: Differential synaptic depression mediates the therapeutic effect of deep brain stimulation
a, Timeline of the experiment: Bilateral AAV injections followed by bilateral fiber implantations in the STN, unilateral 6-OHDA injection into the MFB, and simultaneous bilateral (or, in some cases, unilateral, depending on the quality of GCaMP6f and tdTomato signals) fiber photometry recordings at one week before, and one, two, and three weeks after the 6-OHDA lesion in Vglut2-cre mice. b, Schematic illustration showing the microinjection of AAV to express GCaMP6f and tdTomato in STN neurons. c, Summary of unilateral 6-OHDA lesion-induced changes in intracellular Ca2+ levels in STN neurons on the lesioned and non-lesioned sides. * p < 0.05 (Post-lesion week 2, p = 0.0441; Post-lesion week 3, p = 0.0137), unpaired two tailed t-test. All data are plotted as mean ± SEM. n = 7, 6, and 6 for the post-lesion weeks 1, 2, and 3, respectively, on the 6-OHDA-lesioned side; n = 9, 8, and 8 for the post-lesion weeks 1, 2, and 3, respectively, on the non-lesioned side, from a total of 9 mice including both males and females.
