Extended Data Fig. 10: Comparison of the effects of monophasic and biphasic DBS on Ca²⁺ levels in afferent presynaptic terminals and in STN neurons of normal mice.
From: Differential synaptic depression mediates the therapeutic effect of deep brain stimulation
a, c, f, h, Diagram showing the waveform of the stimulation pulses in monophasic DBS (a, f) and biphasic DBS (c, h). b, d, Time-lapsed ratio of GCaMP6f/tdTomato fluorescence (normalized to the baseline before the first DBS stimulation) to show the DBS-induced responses in presynaptic terminals in the STN with monophasic DBS (b) and biphasic DBS (d). g, i, Time-lapsed ratio of GCaMP6f/tdTomato fluorescence (normalized to the baseline before the first DBS stimulation) to show the DBS-induced responses in the STN neurons with monophasic DBS (g) and biphasic DBS (i). e, j, Summary of DBS-induced changes in presynaptic terminals in the STN (e) and in STN neurons (j). All data are plotted as mean ± SEM. n = 9 for presynaptic group from 3 mice with 3 repetitions (e), and n = 15 for postsynaptic group from 5 mice with 3 repetitions (j), including both males and females, unpaired two tailed t test.
