Extended Data Fig. 9: Regional m6A patterns in disorder-risk genes.
A) Brain-region–specific m6A marked genes are more highly enriched for brain-related diseases than uniformly methylated genes according to DisGeNET analysis. P-values were adjusted for multiple testing using the Benjamini–Hochberg procedure to control the false discovery rate, and associations with FDR < 0.05 are considered statistically significant. (B) Boxplots showing brain-region–specific m6A levels of genes known to be associated with each disease/disorder. Brain regions most affected by the particular disorder tend to show higher m6A methylation rates in those associated genes than other brain regions tested. Each boxplot shows the number of genes with brain-region—specific m6A linked to each disease: Alzheimer’s disease (57), Parkinson’s disease (28), intellectual disability (90), ADHD (21), schizophrenia (91), bipolar disorder (46), epilepsy (61), and depression (33). Boxes extend from the 25th percentile (Q1) to the 75th percentile (Q3) of the relative errors, with the median indicated by a horizontal line inside each box. Whiskers indicate the minimum and maximum values within 1.5x the interquartile range (IQR) from the box. Differences between pairs of groups were assessed using a two-sided Wilcoxon-test, with a total of four pairwise comparisons performed. P-values are indicated on the plot. NS = not statistically significant.
