Extended Data Fig. 6: Characterization of iPSC-derived cells by comparison with published datasets, and functional characterization for transporter expression and TEER in iECs.
a-c. Heatmap showing abundance of expression levels comparing iPC-pPC (a), iSMC-pSMC (b) and iAS-pAS (c) to published datasets31,32. d. PCA based on all transcripts detected in iEC, iPC and iSMC compared to published datasets33,34. EC subtypes present in the published data have been grouped into one general EC group for simplification. e. Relative RNA abundance of ABC and SLC transporters in iEC measured by bulk-RNA sequencing (n = 5 replicates) normalized to whole group average (z-score) to show relative expression. f. Relative RNA expression of inflammation markers in iEC after TNFα treatment normalized to EMC7 (n = 6 replicates/sample; normalized to NT condition; two-tailed T-test, P < 0,0001). g. Representative immunohistochemistry of iEC after non-treated (NT) and TNFα treatment stained for ICAM1 and E-selectin with the corresponding quantification of mean intensity (n = 15 replicates/sample; normalized to NT condition; two-tailed T-test, P < 0,0001). h. TEER of iEC after TNFα treatment (relative to NT condition) (n = 3 replicates/sample; normalized to NT condition; one-way ANOVA corrected for multiple comparisons by Tukey’s method, P = 0,001). The TEER graphs show all three measurements (same symbol) from each replicate (different symbol) to indicate low variability. Bar graphs show mean ± SD. Box and whisker plots show median, lower and upper quartile and all data points.
