Extended Data Fig. 9: Caveolae enrichment and cell junction reduction induced by endothelial FOXF2 deficiency in the mouse.

a. Representative immunohistochemistry of mouse brain slices stained for CAV1 (right) and TJP1 (left) with quantification of mean intensity (n = 19–20 replicates/sample; normalized to Ctrl; two-tailed T-test, P =0.038, P<0.0001. Scale bar 25 µm. b. Immunogold localization of CAV1 in KO endothelium using electron microscopy (n = 1 replicate/sample). Scale bar 0.5 µm. Bar graphs show mean ± SD.