Extended Data Fig. 10: FOXF2 loss induces barrier deficits in 2D and 3D cultures. | Nature Neuroscience

Extended Data Fig. 10: FOXF2 loss induces barrier deficits in 2D and 3D cultures.

From: A fully iPS-cell-derived 3D model of the human blood–brain barrier for exploring neurovascular disease mechanisms and therapeutic interventions

Extended Data Fig. 10: FOXF2 loss induces barrier deficits in 2D and 3D cultures.The alternative text for this image may have been generated using AI.

a. Dextran 10-KDa, 40-KDa and 70KDa leakage after 1 h and 3 h in WT and FOXF2KO iECs measured by fluorescent intensity (normalized to WT) (n = 5-6 replicates/group; normalized to WT; one-way ANOVA corrected for multiple comparisons by Tukey’s method, P < 0,001, P=0.03). b. Dextran 40-KDa and 70-KDa leakage after 40 min in WT and FOXF2KO 3D cultures together with representative live imaging pictures. Scale bar 50 µm (n = 10-12 replicates/group; normalized to WT; two-tailed T-test, P =0.0049, P=0.0015). Bar graphs show mean ± SD.

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