Extended Data Fig. 8: Operant learning performance, reaction time analysis, and open-field locomotion analysis.
From: Patterned wireless transcranial optogenetics generates artificial perception
(a) Number of sessions of 100 trials each across three levels of the task to reach the criterion of 80% success rate for animals expressing ChrimsonR (session median Level 1-12, Level 2-6, Level 3-12). One-way ANOVA, p = 0.5750, F (2, 28) = 0.5645; Sidak’s multiple comparisons test, Level 1 vs Level 2, p = 0.7258; Level 2 vs Level 3, p = 0.7596; Level 1 vs Level 3, p > 0.9999; Level 1, n = 11 animals; Level 2, n = 10 animals; Level 3, n = 10 animals. Data are presented as mean ± s.e.m. Dots represent individual animals. (b) Summary data showing the total training length to complete Levels 1-3 and the number of sessions per day. Dark orange, group average; pale orange, individual trajectories; symbols mark the day individual animals reached criterion. (c) Timeline from water restriction to the end of training. Open-field locomotion was measured at the start of water restriction, before device implantation, and 2, 5, and 10 days post-implantation. (d) Left, average movement speed in an open-field arena across days, one-way ANOVA, F (1.739, 5.217) = 1.632, p = 0.2773. Middle, same as left, but for acceleration, one-way ANOVA, F (2.154, 6.461) = 2.358, p = 0.1693. Right, same as left, but for exploration rate, one-way ANOVA, F (1.497, 4.491) = 3.271, p = 0.1354. Box plots show median (line), 25th and 75th percentiles (bounds of box), minimum and maximum values (whiskers). n = 4 animals. (e) Scatter plots showing reaction times in all trials for the Level 3 task as a function of total cortical distance between stimulated digits. (f) Summary data of cumulative distributions of reaction times for all animals.
