Extended Data Fig. 2: Validation of CRISPR screen results by single gene knock-out.
From: In vivo CRISPR screen reveals regulation of macrophage states in neuroinflammation
a, Representative flow cytometry gating to assess gene KOs in Hoxb8FL-derived myeloid cells. b, Representative flow cytometry plots of myeloid cell polarization for Ifngr1-KO, Tnfrsf1a-KO, Tgfbr1-KO and Csf2ra-KO with their intra-animal control. c-f, Percentage of iNOS+ (c, e) and Arg1+ (d, f) cell polarization for Il4ra-KO and the downstream transcription factor Stat6-KO (c, d) and Il10ra-KO (e, f) compared to control (barplot). On the right, scheme of the cytokine signaling pathways, color-coded for the phenotype of the gene KO in M-iNOS (c, e) and M-Arg1 (d, f) polarization derived from the CRISPR screen shown in Fig. 1e. N = 8 animals for Il4ra-KO transfer, n = 5 animals for Stat6-KO transfer, n = 8 animals for Il10ra-KO transfer. Barplots two-tailed paired t-test in (c-f), ns p-value > 0.05, * p-value < 0.05, ** p-value < 0.01, *** p-value < 0.001, **** p-value < 0.0001; (c-f) schemes stars indicate p-value and fdr < 0.05 and absolute log2(Fold Change) > 3 x sd of noise distribution (see Methods for details).
