Extended Data Fig. 4: ECM depletion increases synapse density in the adult brain.
From: Extracellular matrix proteolysis maintains synapse plasticity during brain development
a) Representative images and quantification of Chat-PSD95FingR at 60 dpf from fixed section stained for ΤdT six hours after vehicle (PBS) or hyaluronidase injection. Dots represent means per fish, with n = 1-4 dendritic segments quantified per fish (vehicle, n = 16 dendrites from n = 10 fish; Hyal, n = 20 dendrites from n = 13 fish; p = 0.018*, Welch’s t-test for fish). Scale: 5 µm. b) Representative images and quantification of Chat-PSD95FingR dendrites at 60 dpf from fixed section stained for ΤdT from bcan+/+vs. bcan−/− fish. Dots represent means per fish, with n = 1 ~ 3 dendritic segments quantified per fish (bcan+/+, n = 25 dendrites from n = 13 fish, bcan−/−, n = 11 dendrites from n = 7 fish; p = 0.048*, Welch’s t-test for fish). Scale: 5 µm. Values were plotted as mean ± SEM. *: p < 0.05.
