Extended Data Fig. 6: mmp14b is expressed in microglia and astrocytes, but not neurons.
From: Extracellular matrix proteolysis maintains synapse plasticity during brain development
a) Representative image of RNA in situ hybridization for mmp14b in mpeg-GFP fish. Dashed lines indicate hindbrain regions. White box indicates inset in b. Similar result was observed from n = 4 fish from single experiment. Scale: 100 µm. b) Inset of microglia in a. Arrowheads indicate mmp14b puncta in mpeg-GFP microglia. Dashed lines indicate microglia. Scale: 5 µm. c) Quantification of percentage of hindbrain microglia expressing mmp14b. Dots indicate n = 4 fish. d) Representative images of RNA in situ hybridization for mmp14b in astrocyte reporter line Tg(slc1a3b:myrGFP-P2A-H2A-mCherry) at 14 dpf. The mCherry signals indicate the astrocyte nucleus. Dashed lines indicate hindbrain regions. Inset is shown at the bottom. Similar result was observed from n = 3 fish from single experiment. Scale: 100 µm. e) Representative images of RNA in situ hybridization for mmp14b in neuronal reporter line Tg(NBT:dsRed) at 14 dpf. Dashed lines indicate hindbrain regions. Inset is shown at the bottom. Similar result was observed from n = 3 fish from single experiment. Scale: 100 µm. f) Representative image of RNA in situ hybridization for mmp14b in mpeg-GFP;mmp14b−/− fish showing lack of probe hybridization. Dashed lines indicate hindbrain regions. Similar result was observed from n = 3 fish from single experiment. Scale: 100 µm. Values were plotted as mean ± SEM.
