Extended Data Fig. 9: Effect of forced swim paradigm on synapses and brevican.
From: Extracellular matrix proteolysis maintains synapse plasticity during brain development
a) Non-merged images of time lapse imaging experiment from Fig. 6g. Circles indicate stable synapses. Boxes indicate the region shown in Fig. 6g. Scale: 5 µm. b) Non-merged images of time lapse imaging experiment from Fig. 6m. Circles indicate stable synapses. Boxes indicate the region shown in Fig. 6m. Scale: 5 µm. c) Representative images and quantification of brevican staining in control and forced swim groups at 14 dpf, with microglia ablation. (CT, n = 18 fish; Mtz, n = 14 fish; p = 0.706, Welch’s t-test). Tg(mpeg:gal4);Tg(UAS:NTR-mCherry) fish was treated with 5 mM Mtz during the forced swim paradigm. Brevican intensity normalized to the mean of the control group. Scale: 20 µm. d) Representative images and quantification of brevican staining in control and forced swim groups at 14 dpf, without microglia ablation. (CT, n = 14 fish; Mtz, n = 14 fish; p = 0.004**, Welch’s t-test). Non-transgenic siblings were treated with 5 mM Mtz during the forced swim paradigm. Brevican intensity normalized to the mean of the control group. Scale: 20 µm. e) Representative images and quantification of synapse density per µm of dendrite length (Chat-PSD95FingR puncta) in bcan−/− fish from forced swim and control group at 14 dpf. Dots represent means per fish from n = 1-3 dendritic segments analyzed per fish (CT, n = 10 dendrites from n = 8 fish; FS, n = 10 dendrites from n = 7 fish; p = 0.732, Welch’s t-test for fish). Scale: 5 µm. Values were plotted as mean ± SEM. **: p < 0.01; ns: not significant.
