Fig. 5: Brevican and MMP14 regulated neuromotor behaviors.
From: Extracellular matrix proteolysis maintains synapse plasticity during brain development
a, Schematic of light startle paradigm performed in this study. For the behavior assay, 14-dpf pigmented fish were used. ITI, inter-trial interval. b, Quantification of total distance moved before light stimulus of Trial 1 in the bcan−/− versus bcan+/+ control (bcan+/+, n = 120 fish; bcan−/−, n = 128 fish; **P = 0.011, Welch’s t-test). c, Quantification of maximum (max) velocity before light stimulus of Trial 1 in the bcan−/− versus bcan+/+ control (bcan+/+, n = 120 fish; bcan−/−, n = 128 fish; *P = 0.013, Welch’s t-test). d, Quantification of total distance moved before light stimulus of Trial 1 in the mmp14b−/− versus mmp14b+/+ control (mmp14b+/+, n = 118 fish; mmp14b−/−, n = 52 fish; P = 0.072, Welch’s t-test). e, Quantification of maximum velocity before light stimulus of Trial 1 in the mmp14b−/− versus mmp14b+/+ control (mmp14b+/+, n = 118 fish; mmp14b−/−, n = 52 fish; P = 0.596, Welch’s t-test). f, Representative heatmaps of Trial 1 and Trial 5 from mmp14+/+ and mmp14−/− fish. The heatmap shows the time spent in each area during the light stimulation period. g, Maximum velocity for each 2-s time bin was plotted from 10 s before the first light stimulus to the end of the last 2-s dark period. The results from the Trial 1 and Trial 5 are shown (mmp14b+/+, n = 115 fish from Trials 1–3, n = 75 fish from Trial 4–5; mmp14b−/−, n = 50 fish from Trials 1–3, n = 27 fish from Trials 4–5). h, Quantification of maximum response upon light stimuli for each trial (mmp14b+/+, n = 115 fish from Trials 1–3, n = 75 fish from Trials 4–5; mmp14b−/−, n = 50 fish from Trials 1–3, n = 27 fish from Trials 4–5, ****P < 0.0001, two-way ANOVA, P value for genotype). Values were plotted as mean ± s.e.m. ****P < 0.0001; **P < 0.01; *P < 0.05. Illustration in a created using BioRender.com.
