Extended Data Fig. 2: Behavioral phenotype and covariate characterization of CC083 and CC025 mice.
From: Genetic mapping identifies Homer1 as a developmental modifier of attention
Related to Fig. 2. a, Startle response, measured as the magnitude of the startle amplitude (V) in three CC lines with the Chr13 QTLWSB (low-performing) diplotype, CC025 (n = 7 M + 7 F), CC035 (n = 6 M + 3 F), and CC038 (n = 6 M + 6 F), and three CC lines with the Chr13 QTLB6 (high-performing) diplotype, CC002 (n = 6 M + 6 F), CC051 (n = 6 M + 6 F), and CC083 (n = 7 M + 7 F), two-sided Welch-corrected t-test between haplotypes p < 0.001. Upper and lower box limits indicate 75th and 25th percentiles, centerline indicates the median, upper and lower whiskers are the maximum and minimum data points. No significant interaction between sex and CC line by two-way ANOVA. b-d, Correlations in three CC lines with the Chr13 QTLWSB (low-performing) diplotype, CC025 (n = 7 M + 7 F), CC035 (n = 6 M + 3 F), and CC038 (n = 6 M + 6 F), and three CC lines with the Chr13 QTLB6 (high-performing) diplotype, CC002 (n = 6 M + 6 F), CC051 (n = 6 M + 6 F), and CC083 (n = 7 M + 7 F) between (b) startle response and PPI, measured as percent inhibition, at 3 dB (PP3), 6 dB (PP6), and 12 dB (PP12) above background, (c) weight and startle response, and (d) weight and PPI. e, Auditory brainstem response measured as minimum thresholds in CC025 (n = 3 M + 1 F) and CC083 (n = 2 M + 1 F) as sound pressure level (dB) in response to increasing frequencies (4, 8, 16, 32 kHz). f, Motor coordination measured in CC025 (n = 10 M + 12 F) and CC083 (n = 10 M + 12 F) as latency (s) to fall from the rod in the Rotarod test averaged across 4 consecutive trials. Significant difference between sexes but no significant interaction between sex and CC line by two-way ANOVA. g, Gross motor activity measured in CC025 (n = 11 M + 12 F) and CC083 (n = 11 M + 12 F) mice as total distance moved (inch) in a square open field arena during a 5-min test. Significant difference between sexes but no significant interaction between sex and CC line by two-way ANOVA. h-i, Performance of CC025 (n = 8 M + 10 F) and CC083 (n = 9 M + 12 F) mice during nosepoke shaping, where the motor activities required are the same as the SDT training but with no attentional component (that is cue), showing (h) the number of nosepokes per mouse on the day of nosepoke shaping when the mice met criteria to proceed to SDT training and (i) the average latency to nosepoke after retrieving a reward for each mouse that retrieved rewards on the first nosepoke shaping day. No significant interaction was found between sex and CC line by two-way ANOVA. j, Working memory performance assessed during a spontaneous alternation task in a Y-maze apparatus for CC025 (n = 9 M + 12 F) and CC083 (n = 13 M + 12 F) mice, measured as the percent of correct alternations (Methods). No significant interaction between sex and CC line by two-way ANOVA. k, Short-term memory tested by a novel object recognition test in CC025 (n = 9 M) and CC083 (n = 10 M) mice, measured as time spent exploring the novel object vs the familiar one and expressed as the percentage of total exploration time during a 5 min test. Two-way ANOVA showed a significant main effect for novelty (p < 0.0001), but not for CC line. l, Social behavior for CC025 (n = 9 M + 7 F) and CC083 (n = 8 M + 7 F) mice, expressed as discrimination index determined by exploration time in a 3-chamber social interaction test. Significant difference between sexes but no significant interaction between sex and CC line by two-way ANOVA. m, Anxiety-like behavior measured as time, in seconds, spent in the center of an open field arena during a 5 min test in CC025 (n = 8 M + 10 F) and CC083 (n = 11 M + 10 F). Significant main effects for CC line (p = 0.0007) and sexes (p = 0.0005) but no significant interaction between sex and CC line by two-way ANOVA. n, Anxiety-like behavior measured as the percentage of time spent in the open arm of an elevated plus maze during a 5-min test in CC025 (n = 9 M + 10 F) and CC083 (n = 11 M + 12 F) mice. Significant main effect for CC line (p = 0.0006) but no significant sex effect or interaction between sex and CC line by two-way ANOVA. Data in e-n are shown as mean ± SEM.
