Extended Data Fig. 5: vLGN neurons inhibit LHA GABAergic neurons through direct projections. Related to Fig. 4.
a, Left, scheme for specific labeling of LHA-projecting vLGN neurons with eGFP. Right, representative images of the vLGN and LHA 2 weeks after virus injections, in which the location of the injection site of rAAV2/2-Retro-Cre was visualized with CTB-647. Scale bar, 200 μm. b, A representative example of LHA-projecting vLGN neurons labeled with eGFP (number indicate distance from bregma). Scale bar, 200 μm. c, Left, representative images of the vLGN illustrating eGFP-expressing vLGN neurons co-labeled with GABAergic neurons. Right, pie chart indicates percentage of eGFP-expressing vLGN neurons co-labeled with GABAergic neurons; n = 5 mice. Scale bar, 50 μm. d, Left, scheme for specific labeling of LHA neurons receiving direct vLGN inputs with eGFP. Right, representative images of injection site of vLGN and LHA. Scale bar, 200 μm. e, Representative example showing LHA postsynaptic neurons with eGFP. Scale bar, 200 μm. f, Left, a representative images of LHA illustrating eGFP-expressing LHA neurons co-labeled with GABAergic neurons. Right, pie chart indicates percentage of eGFP-expressing LHA neurons co-labeled with GABAergic neurons; n = 5 mice. Scale bar, 50 μm. g, Scheme showing specific unilateral infection of vLGN GABAergic neurons with eGFP using vGAT-Cre mice. Scale bar, 200 µm. h, Representative images showing the downstream areas projection by vLGN GABAergic neurons. Scale bar, 200 µm. i, Labeling of vLGN GABAergic neurons with DIO-ChR2 and LHA GABAergic neurons with DIO-eGFP in vGAT-Cre mice. j, Representative images of LHA GABAergic neurons and fiber connections from vLGN GABAergic neurons. Scale bar, 200 μm (Left) and 50 μm (Right). k, Optogenetically evoked IPSCs in LHA GABAergic neurons expressing eGFP; n = 38 cells from four mice.
