Extended Data Fig. 4: Site-specific anti-capsid antibodies less effectively inhibit tau binding to HSV1. | Nature Neuroscience

Extended Data Fig. 4: Site-specific anti-capsid antibodies less effectively inhibit tau binding to HSV1.

From: Phosphorylated tau exhibits antimicrobial activity capable of neutralizing herpes simplex virus 1 infectivity in human neurons

Extended Data Fig. 4: Site-specific anti-capsid antibodies less effectively inhibit tau binding to HSV1.

Synthetic 2N3R tau and 2N4R GSK-3β p-tau isoforms were incubated with heat-immobilized HSV1 capsid after pre-incubation with anti-capsid protein antibodies in an indirect ELISA to examine inhibition of tau-capsid binding. (a) 2N3R tau binding to HSV1 capsid was pretreated with anti-ICP5 antibody. (b) 2N4R GSK-3β p-tau binding to HSV1 capsid was pretreated with anti-RS1-ICP4 antibody (F(8, 32)=13.59, [0 vs. 1.0 μg/mL, **P=0.0064], [0 vs. 5.0 μg/mL, **P=0.0056]). (c) 2N4R GSK-3β p-tau binding to HSV1 capsid was pretreated with anti-pUL25 antibody. Box plots are representative of ±SEM (n=12) depicting median and IQR with whiskers denoting variability according to Tukey’s method. Statistical significance was calculated by two-tailed Mann-Whitney test (a) and one-way ANOVA using Dunnett’s multiple comparison test (b, c).

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