Extended Data Fig. 10: p-tau localizes in dystrophic neurites with HSV1 and around infected nuclei during viral replication. | Nature Neuroscience

Extended Data Fig. 10: p-tau localizes in dystrophic neurites with HSV1 and around infected nuclei during viral replication.

From: Phosphorylated tau exhibits antimicrobial activity capable of neutralizing herpes simplex virus 1 infectivity in human neurons

Extended Data Fig. 10: p-tau localizes in dystrophic neurites with HSV1 and around infected nuclei during viral replication.

3D ReNcell VM cultures were infected with HSV1 to characterize tau’s co-localization with HSV1. (a, b) 3D ReNcell VM cultures were infected with HSV1 for 24 h, immunoprobed with anti-p-tau (PHF-1) labeled with a fluorescent secondary antibody, and analyzed for neurons (GFP), HSV1 (RFP), and p-tau (647) fluorescence by confocal microscopy. (a) Dystrophic neurites, identified by punctate linear formations originating from a cell soma, contain p-tau and HSV1 fluorescence. (b) Increased punctate p-tau fluorescence observed in neuron soma adjacent to HSV1-positive cells and in cells with nuclear replication compartments (arrows). Panels are representative of data from multiple discrete imaging fields across experiments repeated in triplicate.

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