Extended Data Fig. 5: Comparison of mouse and human Rgl cell types and characterization of Rgl1 used for CellChat analysis.

a–c, Gene set enrichment analysis comparing human hFP-Rgl1, hRgl2, and hRgl3 transcriptomes with genes uniquely expressed in mouse Rgl1 (a), mRgl2 (b), and mRgl3 (c). a, hFP-Rgl1: NES = 1.369, FDR < 0.001. hRgl2: NES = −1.115, FDR < 0.001. hRgl3: NES = −1.115, FDR < 0.001. b, hFP-Rgl1: NES = −1.171, FDR = 0.172. hRgl2: NES = 1.524, FDR < 0.001. hRgl3: −1.300, FDR = 0.035. c, hFP-Rgl1: NES = -1.159, FDR = 0.171. hRgl2: NES = −1.330, FDR = 0.018. hRgl3: NES = 1.578, FDR < 0.001. d, Chord diagram showing predicted outgoing interactions from hRgl3 with receptors expressed by hFP-Rgl1. Interactions with a communication probability of >3% are shown. e, UMAP of hRgl1 cells used for CellChat analysis, stratified by midbrain FP marker expression (FOXA2⁺/LMX1A⁺/EN1⁺) and colored by embryonic week. f, Pie chart showing distribution of hFP-Rgl1 and hnonFP-Rgl1 cells used for CellChat analysis based on embryonic week. g, UMAP of mouse Rgl1 (mRgl1) cells used for CellChat analysis colored by embryonic day. h, Pie chart showing distribution of mRgl1 cells used for CellChat analysis based on embryonic day. i, UMAP visualization of ALDH1A1 expression in the developing human VM, based on data from ref. ⁴.